지방이식 for Dummies
지방이식 for Dummies
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Notice: Never centrifuge the Supply Cartridge at this stage as this can cause decline in shipping and delivery performance. Gently tap the Delivery Cartridge numerous moments to collect quantity at the bottom if needed.
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The in vitro differentiation of human pluripotent stem cells (hPSCs) into distinct cell and tissue types allows the review of human biology with no need to have for Principal tissues or in vivo types.
Perform swiftly at this stage to pool wells right into a fifteen mL tube. Notice: For a big-scale dissociation, utilize a multichannel pipette to pool cells into a sterile reagent reservoir. Insert the pooled wells right into a fifteen mL tube.
The STEMdiff™ system offers a standardized method for differentiating hPSCs into epithelial cells that can be later on cultured in second or 3D formats based on the investigation desires.
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Heading back again to the plate, rinse Just about every well with 1 mL of FACS buffer and transfer the amount on the 지방이식 fifteen mL tube. Be aware: Continue to keep cell suspension on ice right after transfer to the tube till willing to operate FACS.
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When coming up with tutorial RNAs, it is suggested to select sequences that align With all the transcription begin web site with the gene of fascination the place achievable.
A minimum amount reaction volume of 50 µL is needed for regular performance With all the CellPore™ Transfection System.
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